2.7 Extraction and quantification of lycopene
Acetone extraction and HPLC quantification method including the mobile phase composition was utilized as previously described with modification as follows. The analytes were separated on an Ultimate XB-C18 (150 mm× 4.6 mm, 3.5 μm, Welch, China), which was kept at 30 °C, and detected at 470 nm using a Shimadzu UV-2550 spectrophotometer (Shimadzu, Kyoto, Japan). The retention times of lycopene was 12 min Standard curves were generated using commercially available reference standards for lycopene (Macklin, China).
3. RESULTS AND DISCUSSION