Funding statement
This work was supported by the Natural Science Foundation of Hunan (2021JJ30986, 2018JJ3728, 2018JJ2596),General Project of Scientific Research Project of Hunan Provincial Health and Family Planning Commission(20200807), Innovation and entrepreneurship education curriculum construction project of Central South University,The Project of New Clinic Techniques of Central South University and National Natural Science Foundation of China(81300600).