Infusion of angiotensin II or aldosterone promotes immune cell
infiltration in the brain
Angiotensin II infusion caused a marked increase in systolic blood
pressure (by ~40 mmHg, Figure 1A). Elevation of blood
pressure had no effect on the number of microglia (Figure 1C), but there
was accumulation of circulating CD45+ leukocytes
(Figures 1B and 1D; gating strategy for other leukocytes are shown in
the Supplementary Figure 1), specifically T cells (Figures 1E and 1F),
myeloid cells (Figure 1G), neutrophils (Figure 1H), monocytes (Figure 1I
and 1J) and B cells (Figure 1L) in the brain. There was also a tendency
for more macrophages (Figure 1K; P =0.15). Similarly, aldosterone
infusion caused elevation of systolic blood pressure (by
~20 mmHg, Figure 2A), without affecting the number of
microglia (Figure 2C). Furthermore, aldosterone also promoted
accumulation of leukocytes (Figures 2B and 2D), including T cells
(Figures 2E and 2F), myeloid cells (Figure 2G) and macrophages (Figure
2H) in the brain.