Assessment of working memory
Working memory was assessed by the novel object recognition test
(Lueptow, 2017). Mice were acclimated for 2 d by placement in the empty
testing box for 10 min. On the day of testing, the mouse was placed in
the same box with two identical objects for 10 min. One h later, the
mouse was placed back in the same box for 5 min with one of the familiar
objects replaced by a novel object. The mouse’s interactions with the
objects (nose point entering a zone 2 cm wide around the object) were
tracked using automated software (Ethovision XT, Noldus Information
Technology BV, Wageningen, The Netherlands).