Figure legends
Figure 1: SARS-CoV-2 CD4+ driven T cell response characterized by low avidity among the PASC subjects. Characterization of SARS‑CoV-2 S-reactive T cells in PASC and control subjects. Blood samples of 17 pediatric PASC patients and 13 convalescent controls were stimulated with SARS-CoV-2 S-WT and analyzed by flow cytometry. (A) Frequencies of WT-reactive CD4+ T cells among PASC and controls. (D) Frequencies of WT-reactive CD8+ T cells among PASC and controls. (B & E) Frequencies of WT reactive CD4+CD3low+ and CD8+CD3low+ T cells. A high functional avidity of SARS-CoV-2 reactive T cells was approached by determining the CD3low+ cells among CD4+CD154+CD137+ and CD8+CD137+ cells. (C & F) Frequencies of WT reactive CD4+CD3high+ and CD8+CD3high+ T cells. A low functional avidity capacity of SAR-CoV-2 reactive T cells was determined by detecting CD3high+ cells among CD4+CD154+CD137+ and CD8+CD137+ cells. Unpaired data were compared with Mann-Whitney-test. P<0.05 was considered significant, only significant p values are documented in the figures.
Figure 2: Analysis of cytokine-producing SARS-CoV-2 reactive T cells. The frequencies of IL2, IFNγ, TNFα or GrB producing WT-reactive T cells were analyzed among PASC subjects and controls. (A & E) IL2 producing SARS-CoV-2 reactive CD4+ and CD8+ T cells. (B & F) TNFα producing SARS-CoV-2 reactive CD4+ and CD8+ T cells. (C & G) IFNγ producing SARS-CoV-2 reactive CD4+ and CD8+ T cells. (D & H) GrB producing SARS-CoV-2 reactive CD4+ and CD8+ T cells.
Figure 3: Analysis of neutralizing capacity of humoral immunity in pediatric PASC compared to controls. Analysis of WT S NAbs titers of both study groups. Scatterplots show line at median. Unpaired data were compared with Mann-Whitney-test. P<0.05 was considered significant, only significant p values are documented in the figures.