Figure 5 Continuous production of scFv-Fc in semi-continuous cultures with high cell density. CHO/GEV_scFv-Fc cells (10 mL) induced with 0.1 µM 4-OHT were seeded in 50-mL bioreactor tubes at cell densities of 0.5 × 107 cells/mL and 1.0 × 107 cells/mL. Cells were counted the day after seeding. The same seeding density was prepared in fresh medium containing 0.1 µM 4-OHT and then reseeded. (A) Viable cell density. (B) Viability. (C) scFv-Fc production concentration. (D) Specific production rate. (E)Glucose concentration. (F) Lactate concentration. Symbols: open circles, 0.5 × 107 cells/mL; closed circles, 1.0 × 107 cells/mL.