Figure 5 Continuous production of scFv-Fc in semi-continuous
cultures with high cell density. CHO/GEV_scFv-Fc cells (10 mL) induced
with 0.1 µM 4-OHT were seeded in 50-mL bioreactor tubes at cell
densities of 0.5 × 107 cells/mL and 1.0 ×
107 cells/mL. Cells were counted the day after
seeding. The same seeding density was prepared in fresh medium
containing 0.1 µM 4-OHT and then reseeded. (A) Viable cell
density. (B) Viability. (C) scFv-Fc production
concentration. (D) Specific production rate. (E)Glucose concentration. (F) Lactate concentration. Symbols: open
circles, 0.5 × 107 cells/mL; closed circles, 1.0 ×
107 cells/mL.