2.7 Gene ontology enrichment analyses
Gene Ontology (GO) was described with GO terms. GO enrichment analysis was implemented by GOseq 1.10.0 in R packages for the PSGs orthologous groups(Young et al., 2010). We analyzed the GO enrichment for five species of the macrotis group, respectively. GO categories were identified at three levels (biological process, molecular function, and cellular component) that were over-represented by PSGs. Enrichment P -values were calculated and all P -values were multi-normalized FDR using the BH (best-hit) approach. Categories with an FDR of less than 0.05 were considered over-represented.