QPCR
cDNA was diluted 1:10 with RNase-free water. The TaqMan or SYBR Green
detection system was used with commercially available probes
(Supplementary Table 3 for TaqMan and Supplementary Table 4 for SYBR
Green). QPCR analysis was conducted using a QuantStudio™ 6 real-time PCR
system (Invitrogen). Results were analysed using QuantStudio™ software
(Life Technologies, Version 1.1). Cycle threshold (Ct) values were
normalised to the housekeeping gene Glyceraldehyde-3-phosphate
dehydrogenase (GAPDH). Relative gene expression was calculated using the
comparative CT method .