Results

C-176 attenuates STING activation in BV2 cells

To assess the efficacy of C-176 in inhibiting STING activation, we pre-treated BV2 microglia-like cells with 0.1-2µM of C-176 followed by an 8-hour incubation with 10-20µg of STING agonist cyclic-di-GMP (Fig 1). Inhibition of c-di-GMP induced STING and TBK1 phosphorylation was observed following incubation with 0.1, 1 and 2µM C-176, with complete inhibition of STING and TBK1 phosphorylation at a concentration of 2μM of C-176 (Fig 1).