Supplementary Figure 2. STING, TBK1 and IRF3 expression 2h
post-TBI and C-176 administration.
Representative images of STING, p-TBK1 and TBK1 protein expression the
ipsilateral cortex and striatum of mouse brains 2h after
controlled-cortical impact modelling of TBI or sham surgery as detected
by western blot (A-B) . Quantification of total STING(C), phospho-TBK1 (D) and total TBK1 (E)protein expression relative to β-actin loading control. No significant
changes in the expression of (C) STING and (D) p-TBK1
were observed following TBI and C-176 administration compared to sham
mice. TBI-mice treated with C-176 had a significantly lower expression
of (E) total TBK1 compared to sham mice (p=0.026). Gene
expression analysis by qPCR revealed no significant changes in the
expression of TMEM173 (F) in the ipsilateral cortex and
striatum and IRF3 (G) in the ipsilateral striatum 2h-post TBI.
TBI-mice treated with C-176 had significantly elevated IRF3 expression
compared to sham (p<00001) and vehicle-treated TBI-mice
(p<0.00001). All data is expressed as mean ± SEM. Significance
determined using a one-way ANOVA with Tukey’s multiple comparison test.
**p ≤ 0.01, *** p ≤ 0.001. n = 7 for each treatment group.