Strains and plasmids Description Reference
Strains
E. coli DH5α General cloning host Our lab
Mycobacterium neoaurum NwIB-HK86 W Starting strain, knocked of kstD1, kstD2, kstD3 Our lab
HK86-K kshA1 over-expressed strain of HK86 W Our lab
HK86-A hsd4A over-expressed strain of HK86 W This study
HK86 A-K hsd4A and kshA1 over-expressed strain of HK86 W This study
HK86 K-A kshA1 and hsd4A over-expressed strain of HK86 W This study
Plasmids
pMV261 Shuttle vector of Mycobacterium and E. coli, carrying the heats shock hsp60 promoter, Kana R Our lab
pMV261-hsd4A Recombinant pMV261, for over-expression of hsd4A in HK86 W This study
pMV261-hsd4A- kshA1 Recombinant pMV261, for over-expression of hsd4A and kshA1 in HK86 W This study
pMV261-kshA1-hsd4A Recombinant pMV261, for over-expression of kshA1 and hsd4A in HK86 W This study