1/ Main results
In this study, we aimed to determine whether sensitization profiles tor children with SRW or SA could be distinguishable from those with NSRW or NSA using a CRD multiplex assay. Although c-sIgE sensitization was frequent, observed in 51% of preschoolers and 75% of school-age children, the patterns of biological source sensitization did not clearly discriminate between preschool children with NSRW and those with SRW or school-age children with NSA and those with SA. At the component level, we observed age-related specificities. Sensitization to airborne allergens, especially towards HDM components, and multi-sensitization, were approximately twice as frequent among preschoolers with NSRW than those with SRW. At school-age, sensitization to some components (ovomucoid, hazelnut 2 S globulin, dog salivary lipocalin proteins, and nsLTP) was more frequent among children with SA and sensitization to nsLTP was associated with impaired lung function. Unsupervised clustering confirmed the heterogeneity in sensitization profiles, identifying three clusters for preschool children and four for school-age children, of different sizes, with shared patterns but also some specificities (grass and PR-10 among preschool children and nsLTP among school-age children). Only one small cluster with multiple airborne and nsLTP sensitization was associated with asthma severity at school-age.