1/ Main results
In this study, we aimed to determine whether sensitization profiles tor
children with SRW or SA could be distinguishable from those with NSRW or
NSA using a CRD multiplex assay. Although c-sIgE sensitization was
frequent, observed in 51% of preschoolers and 75% of school-age
children, the patterns of biological source sensitization did not
clearly discriminate between preschool children with NSRW and those with
SRW or school-age children with NSA and those with SA. At the component
level, we observed age-related specificities. Sensitization to airborne
allergens, especially towards HDM components, and multi-sensitization,
were approximately twice as frequent among preschoolers with NSRW than
those with SRW. At school-age, sensitization to some components
(ovomucoid, hazelnut 2 S globulin, dog salivary lipocalin proteins, and
nsLTP) was more frequent among children with SA and sensitization to
nsLTP was associated with impaired lung function. Unsupervised
clustering confirmed the heterogeneity in sensitization profiles,
identifying three clusters for preschool children and four for
school-age children, of different sizes, with shared patterns but also
some specificities (grass and PR-10 among preschool children and nsLTP
among school-age children). Only one small cluster with multiple
airborne and nsLTP sensitization was associated with asthma severity at
school-age.