Figure 1. AB4 ameliorates DSS-induced colitis symptoms.(A ) Chemical structure of AB4, AA3, and 23-HA. (B-C ) THP-1 cells (pretreated with 500 nM PMA for 3 h) were pretreated with AB4, AA3, and 23-HA (50μM) for 4h, and then cultured with LPS (1μg/ml) for 6h. (B ) The mRNA expression of IL-1β was analyzed by qPCR. (C ) Released IL-1β in the supernatant was analyzed by ELISA. Data are presented as mean ± SD. **P < 0.01 vs. Control group;#P < 0.05, ##P < 0.01 , and ns, not significantly different vs. LPS group. $$P < 0.01 LPS+AB4vs. LPS+AA3. C57BL/6 mice were intraperitoneally injected with AB4 (5, 10, and 15mg/kg) for 7 days, and then given 3.0% DSS in drinking water for 7 days, during which AB4 (5, 10, and 15mg/kg) was continuously injected intraperitoneally (n = 8 per group). At the end of the experiment, the mice were sacrificed, and the colons and the spleens were collected. (D ) The body weight change, (E ) the disease activity index (DAI), (F ) the colon length, (G ) the spleen index, and (H ) the survival rate were measured. Data are presented as mean ± SD. **P < 0.01and ns, not significantly different vs. Normal group;#P < 0.05, ##P < 0.01 vs. DSS group.