Histological analysis and histopathological scores
On the 7th day of modeling, the mice were sacrificed and the colons were taken. The colons were carefully rinsed with PBS solution, and the same parts of each colon were soaked and fixed with 4% paraformaldehyde. Conventional paraffin-embedded sections were stained with hematoxylin and eosin (H&E staining). The histopathological scores were determined using a well-established system (Cui et al., 2020; Lv et al., 2021).