Figure 3. AB4 specifically inhibits NLRP3 inflammasome
activation in colonic macrophages. (A ) Production of IL-1β,
IL-18, IL-6, iNOS, and TNF-α in the serum of DSS-induced colitis mice
was determined by ELISA. (B ) The indicated proteins of colonic
homogenate-related factors were determined by Western Blot. (C )
The mRNA levels of colonic homogenate-related factors were determined by
qPCR. Western Blot was used to detect NLRP3, Caspase-1, and IL-18
protein expression in colon macrophages (D ) and epithelial
cells (E ), and ELISA was used to detect IL-1β protein
expression. Data are presented as mean ± SD. **P < 0.01vs. Normal group; #P < 0.05,##P < 0.01 vs. DSS group.