Histological analysis and histopathological scores
On the 7th day of modeling, the mice were sacrificed and the colons were
taken. The colons were carefully rinsed with PBS solution, and the same
parts of each colon were soaked and fixed with 4% paraformaldehyde.
Conventional
paraffin-embedded
sections were stained with hematoxylin and eosin (H&E staining). The
histopathological scores were determined using a well-established system
(Cui et al., 2020; Lv et al., 2021).