Fig. 10. Evaluation of hesperetin resistance against BPXV
BPXV was sequentially passaged 40 times in Vero cells in the presence of hesperetin or DMSO. For each passage, confluent monolayers of Vero cells were infected with BPXV at an MOI of 0.1, followed by washing with PBS and the addition of fresh DMEM supplemented with hesperetin (3 μg/ml) or 0.05% DMSO. At the end of P40, the fitness of hesperetin- or DMSO-passaged viruses was evaluated again against hesperetin.