Fig. 2. Time-of-addition assay
Confluent monolayers of Vero cells, in triplicates, were infected with BPXV at an MOI of 5, followed by washing with PBS and hesperetin or DMSO treatment at -0.5 hpi, 1 hpi, 6 hpi, 12 hpi, 18 hpi, 24 hpi, 30 hpi and 36 hpi. Supernatants were harvested at 48 hpi, and infectious virions produced in the supernatant at 48 hpi were quantified by plaque assay.