Fig. 9. In ovo antiviral efficacy of hesperetin against
BPXV
(A) Determination of LD50. Indicated
concentrations of hesperetin, in triplicates, were inoculated in 10 day
old embryonated SPF chicken eggs. The viability of the embryos was
examined for up to 5 days post inoculation and the LD50was determined to be 1080.74 µg/egg (Reed-Muench method).
(B) Survival curve. Five-fold serial dilutions of
hesperetin or DMSO were inoculated, in triplicates, in 10 days old SPF
embryonated eggs via the CAM route, followed by infection with BPXV at
100 EID50. The viability of the eggs was examined for
six days post-infection. The EC50 was calculated to be
22.5 µg/egg (C) Pock lesions . Pock lesions on the CAM of
embryonated SPF chicken eggs at day 6 post-BPXV infection are shown.