Fig. 9. In ovo antiviral efficacy of hesperetin against BPXV
(A) Determination of LD50. Indicated concentrations of hesperetin, in triplicates, were inoculated in 10 day old embryonated SPF chicken eggs. The viability of the embryos was examined for up to 5 days post inoculation and the LD50was determined to be 1080.74 µg/egg (Reed-Muench method). (B) Survival curve. Five-fold serial dilutions of hesperetin or DMSO were inoculated, in triplicates, in 10 days old SPF embryonated eggs via the CAM route, followed by infection with BPXV at 100 EID50. The viability of the eggs was examined for six days post-infection. The EC50 was calculated to be 22.5 µg/egg (C) Pock lesions . Pock lesions on the CAM of embryonated SPF chicken eggs at day 6 post-BPXV infection are shown.