Fig. 1 Antiviral efficacy of hesperetin
(A) Cytotoxicity (MTT) assay. The
indicated concentration of hesperetin or an equivalent volume of DMSO
were incubated with cultured Vero cells for 96 h and cell viability was
determined using the MTT assay. (B-D) Antiviral efficacy
against poxviruses. Vero cells, in triplicates, were infected with the
indicated virus at an MOI of 0.1 in the presence of the indicated
concentration of hesperetin or DMSO. The infectious virus particles
released in the supernatant at 72 hpi were titrated using plaque assay.
Virus yield in hesperetin-treated and DMSO-treated cells in BPXV(B), Vaccinia virus (C) and LSDV (D) infected cells is
shown. (E) Virucidal activity. The indicated
concentrations of hesperetin were pre-incubated with BPXV
(107 pfu/ml) at 37℃ for 2 h, and residual infectivity
of BPXV was determined by plaque assay. The results are representative
of three independent experiments. Error bars indicate SD. Pair-wise
statistical comparisons were performed using the Student’s t-test (*** =
P<0.001).