Fig.5. ERK-MNK1-eIF4E signaling is a prerequisite for BPXV
Vero cells, in triplicates, were infected with BPXV at an MOI of 0.1. At 1 hpi, cells were washed with PBS, and fresh medium containing an ERK inhibitor (FR180204: 0.2 μg/ml), MNK1 inhibitor (CGP57380: 0.5 μg/ml) or eIF4E inhibitor (4EGI-1; 0.5 μg/ml) was added. The progeny virus particles released in the infected cell culture supernatant at 48 hpi were quantified by plaque assay. The values are representative of three independent experiments. Error bars indicate SD. Pair-wise statistical comparisons were performed using the Student’s t-test (*** = P<0.001).