3 Results
All 33 SSR loci exhibited polymorphism among the six Oryza types, resulting in the identification of a total of 621 alleles. The genotypic linkage disequilibrium between loci did not reveal significant values (P >0.05), implying the absence of linkage disequilibrium among loci. Among the Oryza types, RM426 was the most variable locus with 61 alleles, whereas RM477 displayed only 5 alleles, with an average of 18.18 alleles per locus (data not shown). The highest genetic diversity was found in O. rufipogon(H E=0.639), while O. nivara recorded the lowest (H E=0.539) (Table S6). Moreover, a high level of private alleles was observed in the O. rufipogon . Overall, the genetic parameters revealed a considerable genetic diversity among all Sri Lankan Oryza lineage (the averageH E of all Oryza types was 0.587, was 1.287, Table S6), which was not evenly distributed across the differentOryza types (the table of allelic frequencies of each population is available upon request).
Tables S6
Considerably low genetic differentiation was observed among cultivatedOryza types indicating a close genetic relationship (Table S5). Initially, inbred, feral, and landraces were collectively clustered as a cultivated rice group, given the observed genetic similarity among these subgroups. Further, wild rice types, such as O. nivara andO. rufipogon , were categorized as the wild rice group, based on their distinct genetic differentiation. Additionally, the weedy rice populations were classified as the weedy rice group.