Quantification of HBsAg
The supernatant of the cultured cells was harvested at 48 h
post-treatment. Levels of hepatitis B surface antigen (HBsAg) in the
supernatant were quantified using an HBsAg quantitative determination
kit (Shenzhen New Industries Biomedical Engineering Co., Ltd., Shenzhen,
China) on a MAGLUMI X3 series automatic chemiluminescence immunoassay
analyzer according to the manufacturer’s instructions.