H3K4me3 and H3K9me3 deposition on integrated HBV-DNA correlates with viral transcription. (A) Distribution of H3K4me3 and H3K9me3 along the HBV genome in different samples. (B-C) Pearson correlation was used to evaluate the association between histone PTMs H3K4me3 and H3K9me3 on integrated HBV DNA and its transcription levels. The horizontal axis represents histone modification levels of each HBV integrated site, which were represented by taking the log2 ratio of Chip-seq RPM/input RPM. The vertical axis represents the transcription levels of each integrated site, which were represented by taking the log2 of the count of HBV chimeric reads from each integrated site. (D) The green and blue lines represent the ChIP-seq average signal for histone PTM within 5kb upstream and downstream of the integration site, the with and without transcriptional activity, respectively. (E) Pearson correlation between average histone ChIP-seq signal of each integration site (5 kb upstream and downstream) and its corresponding transcriptional activity. The vertical axis represents the transcription levels of each integrated site, which were determined by computing the log2 value of the counts of reads from HBV chimeric reads from each respective integration site.