2.4.2 Data processing
Bacterial 16S rRNA gene sequences were analysed using the Quantitative
Insights Into Microbial Ecology (QIIME) platform. First, the
bidirectional sequences were spliced, the sequences with matching errors
were checked, the chimeric sequences were removed, and high-quality
sequences were obtained for the next analysis. According to UCLUST, all
gene sequences were clustered according to 97% similarity, singleton
OTUs were removed, and a representative OTU sequence was obtained. The
species information was obtained by comparing the representative OTU
sequences with the RDP reference database.