Management of virus-specific IgG data
All EGEA1 and EGEA2 samples were analyzed at the same time. Figure S1 shows a workflow representing the different steps of the management of IgG variables.
Fluorescence intensities (FI) of three replicated spots were measured and the median value of triplicate measurements was calculated (raw data). A calibration of raw data was performed, considering the calibrator and sample diluent estimated in each analysis run (calibrated data).
To determine the analytical sensitivity for each antigen, the background signals of all sample diluent repetitive measurements (n=21) were calculated as a mean FI values + 3 standard deviation for each antigen. Values below this background signal were zero (corrected data). For RV-specific IgG data, summary RV-A, RV-B and RV-C variables were calculated by the sum of IgG responses to 18, 9 and 10 specific peptides respectively, as listed in Table S1.