Ref. Copepod consumers Dietary source Assigned 18S reads 18S prey reads Avg. prey reads per sample Fragment (bp) [Blocking primer] per [DNA] Bias Requirements Suitable consumer characteristics
This study (pilot) O. similis, M. norvegica, Microcalanus spp. Whole bodies 284 212 399 1 189 397 16 000 18S V7 (~100-110) No PCR primers, CNV Upscaled sequencing Any invertebrate
This study (full) O. similis, M. norvegica, Microcalanus spp. Whole bodies 4 268 371 437 52 165 786 120 000 18S V7 (~100-110) No PCR primers, CNV Upscaled sequencing Any invertebrate
Hirai et al., (2018) Calanus sinicus Dissected guts 2 919 386 106 266 2 678 18S V9 (~130) No PCR primers, CNV Dissection Dissectible invertebrates
Yeh et al., (2020) Calanus finmarchicus Dissected foregut NA NA NA 18S V4 (NA) No PCR primers, CNV Dissection Dissectible invertebrates
Cleary et al., (2017) Calanus glacialis Whole bodies †† 11 266 639 638 231 7 975 18S V7 (~250) 20 µM PNA per ~0.5 ng µL-1 DNA PCR primers, CNV, blocking primers PNA design and costs Any invertebrate whose target sequence is known
Cleary et al., (2016)
P. newmani,
P. acuspes Whole bodies NA 28 456 618 18S V7 (~250) 20 µM PNA per ~0.5 ng µL-1 DNA PCR primers, CNV, blocking primers PNA design and costs Any invertebrate whose target sequence is known
Ho et al., (2017)
Calanus sinicus
Dissected anterior digestive tracts
††† 2 183 773
NA
NA
18S V4 (~300-350)
2 mM PNA per ~1.7 ng µL-1 DNA
PCR primers, CNV, blocking primers
PNA design and costs, dissection
Dissectible invertebrates whose target sequence is known
Novotny et al., (2021)
Temora longicornis, Acartia spp., Pseudocalanus spp., Centropages hamatus
Whole bodies
NA (>37 mill. 16S + 18S reads)
NA
NA
18S V4 (~400)
Yes, NA [PNA] per NA [DNA]
PCR primers, CNV, blocking primers
PNA design and costs
Any invertebrate whose target sequence is known
Zamora-Terol et al., (2020)
T. longicornis, Acartia spp., Pseudocalanus spp., C. hamatus, Eurytemora affinis
Whole bodies
12 800 000
1 200 000
NA
18S V4 (~400)
Yes, NA [PNA] per NA [DNA]
PCR primers, CNV, blocking primers
PNA design and costs
Any invertebrate whose target sequence is known