This study (pilot) |
O.
similis,
M. norvegica,
Microcalanus spp.
|
Whole bodies |
284 212 399 |
1 189 397 |
16
000 |
18S V7 (~100-110) |
No |
PCR primers, CNV |
Upscaled sequencing |
Any invertebrate |
This study
(full)
|
O. similis,
M.
norvegica, Microcalanus spp.
|
Whole bodies |
4 268 371
437 |
52 165 786 |
120 000 |
18S V7 (~100-110) |
No |
PCR primers, CNV |
Upscaled sequencing |
Any invertebrate |
Hirai et al., (2018) |
Calanus sinicus |
Dissected guts |
2 919
386 |
† 106 266 |
2 678 |
18S V9
(~130) |
No |
PCR primers, CNV |
Dissection |
Dissectible invertebrates |
Yeh et al., (2020) |
Calanus finmarchicus |
Dissected foregut |
NA |
NA |
NA |
18S V4
(NA)
|
No |
PCR
primers, CNV |
Dissection |
Dissectible invertebrates |
Cleary et al., (2017) |
Calanus glacialis |
Whole bodies |
†† 11 266 639 |
638 231 |
7 975 |
18S V7
(~250) |
20 µM PNA per ~0.5 ng
µL-1 DNA |
PCR primers, CNV, blocking primers |
PNA
design and costs |
Any invertebrate whose target sequence is
known |
Cleary et al., (2016) |
|
|
|
|
|
|
|
|
|
|
P. newmani, |
|
|
|
|
|
|
|
|
|
|
P. acuspes |
Whole bodies |
NA |
28 456 |
618 |
18S V7
(~250) |
20 µM PNA per ~0.5 ng
µL-1 DNA |
PCR primers, CNV, blocking primers |
PNA
design and costs |
Any invertebrate whose target sequence is
known |
|
Ho et al., (2017)
|
Calanus sinicus
|
Dissected anterior digestive tracts
|
††† 2 183 773
|
NA
|
NA
|
18S V4 (~300-350)
|
2 mM PNA per
~1.7 ng µL-1 DNA
|
PCR primers, CNV, blocking primers
|
PNA design and costs,
dissection
|
Dissectible invertebrates whose target sequence is known
|
Novotny et al., (2021)
|
Temora longicornis, Acartia spp., Pseudocalanus
spp., Centropages hamatus
|
Whole bodies
|
NA
(>37 mill. 16S + 18S reads)
|
NA
|
NA
|
18S V4 (~400)
|
Yes, NA [PNA] per
NA [DNA]
|
PCR primers, CNV, blocking primers
|
PNA design and costs
|
Any invertebrate whose target sequence is known
|
Zamora-Terol et al., (2020)
|
T. longicornis, Acartia spp., Pseudocalanus spp.,
C. hamatus, Eurytemora affinis
|
Whole bodies
|
12 800 000
|
1 200 000
|
NA
|
18S V4 (~400)
|
Yes, NA [PNA] per
NA [DNA]
|
PCR primers, CNV, blocking primers
|
PNA design and costs
|
Any invertebrate whose target sequence is known
|