Suppressor assay (SA)
CD4+CD25low responder T cells
(Tresp) from donors with birch pollen and associated hazelnut allergy
were stimulated with autologous Bet- or Cor- loaded mDC (control, ctrl).
iTreg primed by IL-10 DCBet (iTregBet)
or IL-10 DC0 (iTreg0) were added in 1:1 or 1:2
Tresp:iTreg ratios (SA 1:1 / SA 1:2), respectively. Tresp, mDC and iTreg
were stained with different cell proliferation dyes for identification
and assessment of T cell proliferation.