3.4 Effects of empagliflozin on IL-1β-induced senescence in mouse chondrocytes
Senescence analysis was conducted to evaluate the effects of empagliflozin on the IL-1β-induced senescence in mouse chondrocytes by western blot and β-galactosidase Staining Assay. It was found that the levels of senescence markers (P21 and P53) were upregulated by IL-1β treatment, but this effect was largely weakened by empagliflozin (Figure 4A). Besides, β-galactosidase Staining results showed that the proportion of SA-β-Gal-positive cells obviously increased after IL-1β treatment but decreased in the presence of empagliflozin (Figure 4B). These data collectively suggested that empagliflozin exerted the protective role in IL-1β-induced cellular senescence of mouse chondrocytes.