Western blot
After treatment, equal amounts of cells were lysed with RIPA for 60min. The lysates were quantified by BCA kit (Beyotime, China). The samples were then separated with SDS-polyacrylamide gels and transferred into nitrocellulose membranes. After being blocked and cut into proper sections, membranes were incubated with different primary antibodies against GAPDH (Abcam, #ab181602), MMP9 (Abcam, #ab76003), MMP13 (Abcam, #ab39012), COX2 (CST, #12282), INOS (Abcam, #ab3523), P53 (Abcam, #ab90363), P21 (Abcam, #ab215971), NF-κB P65 (CST, #8242), P-NF-κB P65 (Ser536) (CST, #3033), IκB (CST, #4812), and P-IκB (Ser32) (CST, #2859) at 4 °C overnight. The membranes were then incubated with luminesced secondary antibodies and detected with the BioRad System. GAPDH was used as control.