Extraction Process
Aerial components of sixteen distinct plant species were gathered from diverse locations within the Greek periphery. The plant species encompassed Achillea taygetea Boiss. & Heldr., Cistus creticus L., Cistus monspeliensis L., Lavandula stoechasL., Mentha aquatica L., Mentha longifolia L.,Origanum vulgare L., Phlomis cretica C. Presl,Rosmarinus officinalis L., Salvia sclarea L.,Satureja parnassica Heldr. & Sart. ex Boiss., Satureja thymbra L., Sideritis euboea Heldr., Sideritis syriacaL., Stachys spinosa L., and Thymus longicaulis C. Presl. The collected plant specimens were finely ground (using SCIS, Allenwest-Eac ltd) into homogeneous powders and subjected to ultrasound-assisted extraction (UAE). An Elma S 100H (Elmasonic) instrument was employed, utilizing 100% ethyl acetate as the extraction solvent. The extraction process took place for 15 minutes at room temperature, with a plant-to-solvent ratio of 1/10 (w/v). To ensure comprehensive extraction, the procedure was repeated twice for each sample. The ethyl acetate solvent was subsequently evaporated to dryness under reduced pressure, employing a Buchi Rotavapor R-200 rotary evaporator, while maintaining a temperature of 40 °C.