Achillea taygetea
The ethyl acetate extract of A. taygetea demonstrated significant inhibitory effects on obligate anaerobic pathogens, with MIC values ranging from 0.04 mg / ml (P. micra ) to 0.60 mg / ml (F. nucleatum ). The effect on facultative anaerobic streptococci varied, with S. oralis inhibited at 0.60 mg / ml, S. sobrinusinhibited at a minimum of 5.00 mg / ml, and the inhibitory concentrations observed in S. mutans tests were attributed to the effects of DMSO. Among the remaining pathogens listed in Table 1, all except S. aureus (MIC = 2.50 mg / ml) showed resistance to the extract. The MBC values ranged from 0.15 mg / ml (P. micra ) to 5.00 mg / ml (S. aureus ), except for S. sobrinus , which was not eliminated in the test.
In the biofilm plate assay, the tested S. mutans strain showed strong inhibition in biofilm formation at a concentration of 5.00 mg / ml. The lower cutoff value was calculated at an optical density (OD595) of 0.143. In the presence of 2.50 mg / ml of the ethyl acetate extract, biofilm production fell into the C2 category. Figure 2 indicates that lower concentrations did not affect biofilm formation, as all absorbance values were higher than the high cutoff value (OD595 = 0.428).