Origanum vulgare
The ethyl acetate extract demonstrated a broad spectrum of activity against all tested pathogens, particularly the oral bacteria (as shown in Table 7), with a MIC range of 0.04 mg / ml (P. gingivalis ,P. micra ) to 2.50 mg / ml (E. coli ). For the facultative anaerobic bacteria, MIC values varied between 0.15 mg / ml (S. mutans , S. sobrinus , S. oralis ) and 2.50 mg / ml (E. coli ). The minimum bactericidal concentration (MBC) values ranged from 0.08 mg / ml (P. micra ) to 2.50 mg / ml (E. coli , C. albicans ). In comparison to C. albicans andE. coli (MBC = 2.50 mg / ml), all other pathogens were eliminated more effectively, with concentrations ranging from 0.08 mg / ml (P. micra ) to 1.25 mg / ml (E. faecalis ).
In the biofilm plate assay, a moderate reduction in biofilm production was observed, as depicted in Figure 2. The lower cutoff value was established between a concentration of 0.30 mg / ml and 0.60 mg / ml, while high biofilm formation was observed at concentrations of 0.08 mg / ml and below.