Satureja thymbra
The ethyl acetate extract of S. thymbra demonstrated effectiveness against nearly all tested microorganisms, with a mean minimum inhibitory concentration (MIC) range of 0.04 mg / ml (P. micra ) to 1.25 mg / ml (S. mutans , E. faecalis ). For the facultative anaerobic bacteria, MIC values varied from 0.30 mg / ml (S. oralis ) to 1.25 mg / ml (E. faecalis , S. mutans ). The minimum bactericidal concentration (MBC) values of theS. thymbra ethyl acetate extract ranged from 0.08 mg / ml (P. micra ) to 5.00 mg / ml (S. mutans , E. faecalis ). However, no antibacterial effects were observed on E. coli , and no antifungal effects were observed on C. albicans in relation to DMSO effects.
In the biofilm plate assay conducted with the tested strain of S. mutans (Figure 2), the ethyl acetate extract exhibited a moderate inhibitory effect on biofilm formation. Below a concentration of 0.60 mg / ml, the absorbance values correlated with the category of no biofilm production..