Mentha aquatica
Table 5 presents the MIC and MBC values for the ethyl acetate extract ofMentha aquatica . The extract demonstrated efficient reduction of obligate anaerobic bacteria, with inhibitory concentrations ranging from 0.30 mg / ml (P. gingivalis ) to 1.25 mg / ml (F. nucleatum ). With the exception of S. oralis and C. albicans , all other pathogens exhibited slight inhibition, with MIC values ranging from 2.50 mg / ml (S. mutans , E. faecalis ) to 5.00 mg / ml (S. sobrinus , E. coli , S. aureus ). Furthermore, the extract displayed a moderate bactericidal effect onP. micra , P. gingivalis , and S. oralis , with MBC values ranging from 1.25 mg / ml (P. micra ) to 5.00 mg / ml (S. oralis ). When considering the effects of DMSO, no significant bactericidal effect was observed on the other strains.
In Figure 3, it is evident that the biofilm formation potential ofS. mutans is significantly reduced at concentrations up to 1.25 mg / ml. Therefore, concentrations at or above this threshold can be categorized as C1, indicating strong inhibition of biofilm production. A concentration of 0.60 mg / ml still exhibited moderate biofilm production, while concentrations higher than 1.25 mg / ml were categorized as C3, indicating no detectable effect on biofilm formation.