2.5 Double luciferase assay
The circRNAs were built into PSI‐CHECK2 vector, then circRNA and miRNA were cotransfected into 293T cells.Luciferase activities were measured at 48‐hours after transfection using the Dual‐Luciferase Reporter AssaySystem (Promega, Madison, WI) according to themanufacturer’s protocol.Renilla luciferase activity wasnormalized to firefly luciferase activity and expressed as apercentage of the control. All transfection experiments were performed in triplicate.