2.8 Cell culture was transfected with SiRNA.
TLR2SiRNA,TLR4SiRNA and TLR8SiRNA were purchased from Huzhou Hippo Biotechnology Co., LTD, the specific sequence is shown in S1. 6cm cell dishes were taken and 2mL (1~2×105cells) culture medium was added into each well. The culture density reached 70-90% at 37℃ and 5%CO2; Dilute Lipofectamine 3000 (Invitrogen, No.3000015) with Opti-MEM and mix well; Dilute the carrier with Opti-MEM, add P3000, and mix well; Diluted carrier (1:1) was added into the diluted Lipofectamine 3000 and incubated at room temperature for 5 minutes; Add the mixture into the culture medium, shake well and incubate at 37℃ for 3 days.