Figure 2 P.g-stimulated macrophages were recruited to the
kidney and polarized to the M1 phenotype
(A) IHC analysis of F4/80 and CD86 expression in kidney sections. (B)
Western blot analysis showed that the expression level of F4/80 was high
in the P.g group. (C-D, G-H) IF staining of the kidney and
SV40-MES-13 cells for CD86 (Alexa Fluor 594, red), iNOS (Alexa Fluor
594, red) and DAPI nuclei staining (200× magnification; scale bar 100
μm). (E-F) Western blotting was performed to examine the effect of M1
macrophages on the kidneys.