Figure 4 M1 macrophage polarization activates the NF‑κB pathway and the NLRP3 inflammasome in the kidney and GMCs.
(A) IF analysis of NF-κB (P65) expression in kidney sections. (B) Protein levels of IKBα, p-IKBα, P65 and p-P65 in the kidney. (C) mRNA levels of NF-κB in the kidney. (D) IHC analysis of NLRP3 in kidney sections. (E and F) Protein and mRNA levels of NLRP3, ASC, Caspase 1 and IL-1β in the kidney. (G) Live/dead cell staining results. (H) CCK-8 analysis of MΦCM on SV40-MES-13 cells. (I) IF analysis of NF-κB (P65) expression in SV40-MES-13 cells. (J, K) Protein levels of NF-κB, NLRP3, ASC, Caspase 1 and IL-1β in SV40-MES-13 cells. (L, M) NF-κB (P65), NLRP3, ASC, Caspase1 and IL-1β mRNA levels. The data are shown as the means ± SDs from 3 experiments. (200× magnification; scale bar 100 μm) (*P<0.05 , vs. the control group). **P<0.01 , vs. the control group).