Figure 6 MΦCM induces SV40-MES-13 cell infiltration via ferroptosis.
Effects of different concentrations of Fer-1 on SV40-MES-13 cell viability and inflammation. (A) Staining of live/dead cells. (B) CCK-8 results showing the viability of SV40-MES-13 cells treated with Fer-1. (C-E) IHC analysis of GPX4, ACSL4 and xCT expression in SV40-MES-13 cells. (F, H and I) NF-кB (P65), NLRP3, ASC, caspase-1, and IL-1β protein expression in GMCs. (G, J and K) ACSL4, GPX4, xCT, NF-кB, NLRP3, ASC, Caspase-1, IL-1β, NCOA4, GPX4 and PTGS2 mRNA levels in SV40-MES-13 cells. The data are shown as the means ± SDs from 3 experiments. (200× magnification; scale bar 100 μm) (*P<0.05 , vs. the control group). **P<0.01 , vs. the control group).