Figure 2 P.g-stimulated macrophages were recruited to the kidney and polarized to the M1 phenotype
(A) IHC analysis of F4/80 and CD86 expression in kidney sections. (B) Western blot analysis showed that the expression level of F4/80 was high in the P.g group. (C-D, G-H) IF staining of the kidney and SV40-MES-13 cells for CD86 (Alexa Fluor 594, red), iNOS (Alexa Fluor 594, red) and DAPI nuclei staining (200× magnification; scale bar 100 μm). (E-F) Western blotting was performed to examine the effect of M1 macrophages on the kidneys.