Figure 7 MΦCM induces SV40-MES-13 cell ferroptosis via the NF‑κB/NLRP3 pathway
(A) Staining of live/dead cells. (B) The viability of SV40-MES-13 cells. (C-D) NF-кB, NLRP3, ASC, Caspase-1, and IL-1β protein expression in SV40-MES-13 cells. (E, F) NF-кB, NLRP3, ASC, Caspase 1 and IL-1β mRNA levels in SV40-MES-13 cells. (G) ACSL4, GPX4 and xCT protein expression in SV40-MES-13 cells. (H) NCOA4, GPX4 and PTGS2 mRNA levels in SV40-MES-13 cells. (I) MiTo-FerroGreen staining. The data are shown as the means ± SDs from 3 experiments. (200× magnification; scale bar 100 μm) (*P<0.05 , vs. the control group). **P<0.01 , vs. the control group).