Mito-FerroGreen (Fe2+) staining
SV40-MES-13 cells were seeded in 6-well plates at a density of 1
×106 cells/well and cultured overnight at 37 ℃ in a
5% CO2 incubator. Then, SV40-MES-13 cells were cultured with medium
containing 10% FBS and 50% MΦCM for 24 h. After the cells were washed
three times with PBS buffer, Mito-FerroGreen working solution (5 μmol/L)
was added and incubated for 30 min at 37 °C in a 5% CO2 incubator.
After the samples were washed 3 times with PBS buffer, a Carl Zeiss
LSM710 fluorescence microscope was used to obtain images.