Serum analysis
Serum was collected from the blood by centrifugation (4 °C, 4000 rpm, 10
min) and used to measure the activity of BUN and serum creatinine (Scr),
which are kidney injury markers. BUN and Scr levels were measured with
BUN and Scr assay kits (Majorbio Biotechnology (Shanghai) Co., Ltd.,
China).