The use of NF‑κB inhibitors
SV40-MES-13 cells were seeded in 6-well plates at a density of 1 ×
106 cells/well overnight. The cytotoxicity of QNZ
(Sigma, USA) was evaluated by CCK-8 assays. After 30 min of treatment
with 12 μmol QNZ, SV40-MES-13 cells were cultured with medium containing
10% FBS and 50% MΦCM for 24 h. Then, SV40-MES-13 cell viability and
ferroptosis were detected as previously described. Moreover, the NF-κB
signaling pathway and inflammatory factors (IL-6, IL-17 and IL-10) were
detected by RT‒PCR and Western blotting.