The use of ferroptosis inhibitors
SV40-MES-13 cells were seeded in 6-well plates at a density of 1 × 106 cells/well overnight. The cytotoxicity of ferrostatin-1 (fer-1, Sigma, USA) was evaluated by CCK-8 assays. After 4 h of treatment with 5 μmol fer-1, SV40-MES-13 cells were cultured with medium containing 10% FBS and 50% MΦCM supernatant for 24 h. Then, SV40-MES-13 cell viability and ferroptosis were detected as previously described.