Figure 5 Oral administration of P.g induces ferroptosis during CKD in vivo and in vitro
(A-C) IHC analysis of GPX4, ACSL4 and xCT in kidney sections. (D) GPX4, ACSL4 and xCT protein expression in kidney tissue. (E) GPX4, PTGS2 and NCOA4 mRNA levels in kidney tissue. (F-H) IF staining of GPX4, ACSL4 and xCT (Alexa Fluor 594, red) and DAPI nuclear staining (blue) in SV40-MES-13 cells. (I) GPX4, ACSL4 and xCT protein expression in SV40-MES-13 cells. (J) GPX4, PTGS2 and NCOA4 mRNA levels. The data are shown as the means ± SDs from 3 experiments. (200× magnification; scale bar 100 μm) (*P<0.05 , vs. the control group). **P<0.01 , vs. the control group).