Figure 9. Effects of hsa_circ_0072697 overexpression and knockdown on PBMCs in AS patients.
(A) Cell proliferation activity of PBMCs in different groups measured by CCK-8 assay. The hsa_circ_0072697 overexpression group showed significantly reduced cell proliferation activity compared to the other four groups, while the siRNA-hsa_circ_0072697 group showed increased cell proliferation activity.
(B) Apoptosis analysis of PBMCs in different groups by flow cytometry. The hsa_circ_0072697 overexpression group had the highest apoptosis rate, while the siRNA-hsa_circ_0072697 group had the lowest apoptosis rate.
(C) Cell cycle analysis of PBMCs in different groups by flow cytometry. The hsa_circ_0072697 overexpression group showed a decrease in the number of cells in the G1 phase and an increase in the number of cells in the S and G2 phases compared to the control group, while the siRNA-hsa_circ_0072697 group showed completely opposite effects.