Cell apoptosis assay by flow cytometry
In this study, we used flow cytometry to analyze cell apoptosis. Cells were harvested by centrifugation after digestion and washed with cold PBS. They were collected (including cells from culture supernatant) and resuspended in 500 μL of Binding Buffer. Next, we added 5 μL of Annexin V-FITC (AP101, Biotech) and 10 μL of propidium iodide (PI) to each sample, gently vortexed samples, then incubated for 5 mins at room temperature in the dark. Stained cells were then analyzed using a flow cytometer (CytoFLEX, BECKMAN) equipped with appropriate lasers and filters.