Cell apoptosis assay by flow cytometry
In this study, we used flow cytometry to analyze cell apoptosis. Cells
were harvested by centrifugation after digestion and washed with cold
PBS. They were collected (including cells from culture supernatant) and
resuspended in 500 μL of Binding Buffer. Next, we added 5 μL of Annexin
V-FITC (AP101, Biotech) and 10 μL of propidium iodide (PI) to each
sample, gently vortexed samples, then incubated for 5 mins at room
temperature in the dark. Stained cells were then analyzed using a flow
cytometer (CytoFLEX, BECKMAN) equipped with appropriate lasers and
filters.