Figure 9. Effects of hsa_circ_0072697 overexpression and
knockdown on PBMCs in AS patients.
(A) Cell proliferation activity of PBMCs in different groups measured by
CCK-8 assay. The hsa_circ_0072697 overexpression group showed
significantly reduced cell proliferation activity compared to the other
four groups, while the siRNA-hsa_circ_0072697 group showed increased
cell proliferation activity.
(B) Apoptosis analysis of PBMCs in different groups by flow cytometry.
The hsa_circ_0072697 overexpression group had the highest apoptosis
rate, while the siRNA-hsa_circ_0072697 group had the lowest apoptosis
rate.
(C) Cell cycle analysis of PBMCs in different groups by flow cytometry.
The hsa_circ_0072697 overexpression group showed a decrease in the
number of cells in the G1 phase and an increase in the number of cells
in the S and G2 phases compared to the control group, while the
siRNA-hsa_circ_0072697 group showed completely opposite effects.