RT-qPCR
To validate the results of RNA-seq analyses, cDNA was prepared from 15
samples from AS patients and control donors via reverse transcription.
The levels of three up-regulated and three down-regulated DECs were then
assessed in these samples via qPCR using the Universal SYBR Green Master
Mix. The primers that had been designed and synthesized by OG-Biotech
Inc. (Table 1). Relative circRNA expression was assessed via the
2−ΔΔCq approach, and receiver operating characteristic
(ROC) curve analyses were used to differentiate between samples in the
control and AS patient groups.
Table1: Specifific circRNAs primers used for qRT-PCR analysis