Electrophoresis and sequencing
PCR products’ electrophoresis was done on a 1 % agarose gel. Seven
microliters of the PCR product were stained with 3 µL safe green dye and
visualized by a UV transilluminator. The PCR products’ sizes were
estimated according to the migration pattern of a 100 bp DNA ladder. The
PCR product was sequenced from both terminals by the Macrogen sequencing
service in South Korea. The DNA sequence was published in GenBank as
Fowl adenovirus isolate FAdV/Kurdistan/2013 accession number KF601576,
FAdV/Kurdistan/2015 accession number KU060147, and FAdV/Kurdistan/2020
accession number MN967070.