FIGURE LEGENDS
Figure 1. Gross lesions in
infected broilers with fowl adenovirus. A: ecchymotic hemorrhages in the
skeletal muscles, B: Hepatomegaly (right) compared to the normal liver
(left), C: Friable liver with petechial hemorrhage, D: A clinical case
in which there is an enlargement of the liver with small white necrotic
foci and petechial hemorrhagic spots.
Figure 2. The
histopathological section in the liver. A: Moderate lymphocytic and
heterophilic inflammatory infiltrates surrounding the central vein.
Diffuse cytoplasmic vacuolation is observed within remaining
hepatocytes, and there is
widening and
infiltration of sinusoids with lymphocytes, heterophils, and
histiocytes. B: In the previous slide with more magnification, the
nuclei of numerous hepatocytes contained one large basophilic inclusion
body (arrow), a halo is present around the intranuclear inclusion, and
the nucleus membrane was hyperchromatic (H and E stain, X100, X200).
Figure 3.
Histopathological
sections in the liver. A: Several individual necrotic hepatocytes are
scattered within the parenchyma. There is hepatic parenchymal disruption
due to coalescing of randomly distributed foci of degenerated
hepatocytes. These hepatocytes are swollen with hypereosinophilic and
highly vacuolated cytoplasm (H and E stain, X100). (B) Numerous
hepatocyte nuclei contained one large basophilic
inclusion body as
indicated by white
arrows (H and E
stain, X100). C: Lymphocytic and heterophilic infiltration surrounding
and close to a central vein. Diffuse cytoplasmic vacuolation is observed
within remaining hepatocytes. Additionally, there is widening and
infiltration of sinusoids with lymphocytes, heterophils, and histiocytes
(H and E stain, X200). D: Large basophilic intranuclear inclusion bodies
(white arrows). The presence of free RBC is indicated by yellow arrows
(H and E stain,
X400).
Figure 4. Histopathological sections in the testicle.
A: Degeneration of
spermatozoa, spermatid, Sertoli cells, and Leydig cells. In the
interstitial tissue, there is intertubular hemorrhage (H) and large
vacuoles (arrows). B: Dilation of seminiferous tubular lumens containing
necrotic debris and devoid of spermatozoa in some of them
(inset). H and E,
X100.
Figure 5. Agarose gel electrophoresis pattern shows PCR amplification of
1300 bp from hexon gene of Kurdistan FAdV Lane L: 100 bp DNA
ladder. Lane 1 indicates FAV field strain.
Figure 6. Phylogenetic tree of Kurdistan FAdV isolates. Analysis of the
phylogenetic tree according to partial Hexon gene sequence
indicates five clusters. The red circle indicates FAdV/Kurdistan/2013
and FAdV/Kurdistan/2020. The pink circle indicates FAdV/Kurdistan/2015.