Electrophoresis and sequencing
PCR products’ electrophoresis was done on a 1 % agarose gel. Seven microliters of the PCR product were stained with 3 µL safe green dye and visualized by a UV transilluminator. The PCR products’ sizes were estimated according to the migration pattern of a 100 bp DNA ladder. The PCR product was sequenced from both terminals by the Macrogen sequencing service in South Korea. The DNA sequence was published in GenBank as Fowl adenovirus isolate FAdV/Kurdistan/2013 accession number KF601576, FAdV/Kurdistan/2015 accession number KU060147, and FAdV/Kurdistan/2020 accession number MN967070.