FIGURE LEGENDS
Figure 1. Gross lesions in infected broilers with fowl adenovirus. A: ecchymotic hemorrhages in the skeletal muscles, B: Hepatomegaly (right) compared to the normal liver (left), C: Friable liver with petechial hemorrhage, D: A clinical case in which there is an enlargement of the liver with small white necrotic foci and petechial hemorrhagic spots.
Figure 2. The histopathological section in the liver. A: Moderate lymphocytic and heterophilic inflammatory infiltrates surrounding the central vein. Diffuse cytoplasmic vacuolation is observed within remaining hepatocytes, and there is widening and infiltration of sinusoids with lymphocytes, heterophils, and histiocytes. B: In the previous slide with more magnification, the nuclei of numerous hepatocytes contained one large basophilic inclusion body (arrow), a halo is present around the intranuclear inclusion, and the nucleus membrane was hyperchromatic (H and E stain, X100, X200).
Figure 3. Histopathological sections in the liver. A: Several individual necrotic hepatocytes are scattered within the parenchyma. There is hepatic parenchymal disruption due to coalescing of randomly distributed foci of degenerated hepatocytes. These hepatocytes are swollen with hypereosinophilic and highly vacuolated cytoplasm (H and E stain, X100). (B) Numerous hepatocyte nuclei contained one large basophilic inclusion body as indicated by white arrows (H and E stain, X100). C: Lymphocytic and heterophilic infiltration surrounding and close to a central vein. Diffuse cytoplasmic vacuolation is observed within remaining hepatocytes. Additionally, there is widening and infiltration of sinusoids with lymphocytes, heterophils, and histiocytes (H and E stain, X200). D: Large basophilic intranuclear inclusion bodies (white arrows). The presence of free RBC is indicated by yellow arrows (H and E stain, X400).
Figure 4. Histopathological sections in the testicle. A: Degeneration of spermatozoa, spermatid, Sertoli cells, and Leydig cells. In the interstitial tissue, there is intertubular hemorrhage (H) and large vacuoles (arrows). B: Dilation of seminiferous tubular lumens containing necrotic debris and devoid of spermatozoa in some of them (inset). H and E, X100.
Figure 5. Agarose gel electrophoresis pattern shows PCR amplification of 1300 bp from hexon gene of Kurdistan FAdV Lane L: 100 bp DNA ladder. Lane 1 indicates FAV field strain.
Figure 6. Phylogenetic tree of Kurdistan FAdV isolates. Analysis of the phylogenetic tree according to partial Hexon gene sequence indicates five clusters. The red circle indicates FAdV/Kurdistan/2013 and FAdV/Kurdistan/2020. The pink circle indicates FAdV/Kurdistan/2015.