Immunofluorescence assay (IFA)
The SF-21 cells, which infected with a baculovirus for expressing the
pB602L protein, or ASFV infected PAM cells were fixed with 4%
paraformaldehyde. After washing three times with PBS, the cells were
blocked with 5% BSA at 37℃ for 1 h and washed three times with PBS. The
cells were then inoculated with 1:1000 mAbs or a murine anti-pB602L
serum for 1 h, followed by four washes with PBST. The Goat anti-Mouse
IgG (H+L) (Invitrogen, USA) was added at a dilution of 1:2000 and
incubated for 30 min at 37℃. The images were captured in an inverted
fluorescence microscope (EVOS FL, Life, USA).