Mouse anti-pB602L mAb production
To produce anti-pB602L monoclonal antibodies, mice were immunized with
the purified recombinant pB602L protein. Following a commonly used
protocol for producing mAbs in mouse (Petrovan et al., 2019), we finally
produced eight clones of anti-pB602L mAbs and they were designated B2A1,
B2F1, B2D10, B2H10, B2B2, B2D8, B2A3, and B2E12, respectively. These
mAbs were identified as belonging to two subtypes of IgG1 and IgG2a
(Table 2). All of them showed good reactivity with both prokaryoticly
and eukaryoticly expressed recombinant pB602L protein indicated by the
assays of Western blot, iELISA and IFA, respectively (Table 2).