Recombinant baculovirus expressed pB602L protein
The B602L gene of ASFV (Pig/HLJ/2018) was cloned into pFastBac-HTA vector by Genscript Biotech Corporation (Nanjing, Jiangsu, China). The recombinant plasmid pFastBac-HTA-B602L was transformed into E. coli DH10 Bac (WEIDI, Shanghai, China) in LB plates, containing: 50 μg/mL kanamycin, 7 μg/mL gentamicin, 10 μg/mL tetracycline, 100 μg/mL X-gal and 40 μg/mL IPTG, incubated at 37 ° C for 48 h. A white colony was piked and cultured in a liquid culture (containing 50 μg/mL kanamycin, 7 μg/mL gentamicin and 10 μg/mL tetracycline) at 37℃ for 24 h. Bacmid was purified with a DNA Isolation Kit (OMEGA bio-tek, USA) and transfected 25 mL insect cells (Sf-21) with a density of 2.5 × 106 cells/ml. Rescued recombinant baculovirus was used to infected Sf-21 cells for expressing pB602L. IFA was used to identify protein expression.