Immunofluorescence assay (IFA)
The SF-21 cells, which infected with a baculovirus for expressing the pB602L protein, or ASFV infected PAM cells were fixed with 4% paraformaldehyde. After washing three times with PBS, the cells were blocked with 5% BSA at 37℃ for 1 h and washed three times with PBS. The cells were then inoculated with 1:1000 mAbs or a murine anti-pB602L serum for 1 h, followed by four washes with PBST. The Goat anti-Mouse IgG (H+L) (Invitrogen, USA) was added at a dilution of 1:2000 and incubated for 30 min at 37℃. The images were captured in an inverted fluorescence microscope (EVOS FL, Life, USA).