SDS-PAGE and Western blot analysis
The protein sample prepared from empty vector transfected cells, uninduced cells, induced cells, purified pB602L or polypeptides was mixed with loading buffer and boiled for 10 min. The proteins in each sample were separated by 10% gel and stained with Coomassie Brilliant blue R-250 for SDS-PAGE. For Western blot analysis, the gel was blotted to PVDF transfer membrane with an electrophoresis apparatus. The membrane was blocked by 5% Difco Skim Milk for 2 h at room temperature and then incubated with each of the mAbs or a mouse monoclonal anti-GST antibody (Sigma-Aldrich, USA) as primary antibody at room temperature for 1 h. After four washes with Tris-Buffered Saline (TBS) add 0.5% Tween-20 (TBST), the membrane was incubated with IRDye 800CW goat anti-Mouse IgG (H + L) as secondary antibody (LICOR, USA) at room temperature for 1 h, followed by four washes with TBST. Western blot analysis was completed with an Odyssey CLX (LICOR, USA).