Mouse anti-pB602L mAb production
To produce anti-pB602L monoclonal antibodies, mice were immunized with the purified recombinant pB602L protein. Following a commonly used protocol for producing mAbs in mouse (Petrovan et al., 2019), we finally produced eight clones of anti-pB602L mAbs and they were designated B2A1, B2F1, B2D10, B2H10, B2B2, B2D8, B2A3, and B2E12, respectively. These mAbs were identified as belonging to two subtypes of IgG1 and IgG2a (Table 2). All of them showed good reactivity with both prokaryoticly and eukaryoticly expressed recombinant pB602L protein indicated by the assays of Western blot, iELISA and IFA, respectively (Table 2).