Cells, serum samples, and experimental animals
The SP2/0 and Sf-21 cells used in this study were maintained in our laboratory. ASFV (Pig/HLJ/2018) infected porcine alveolar macrophages (PAM), tissues samples including the spleens, tonsils, and gastrohepatic lymph nodes from ASFV infected pigs, and anti-ASFV positive sera were obtained from the National High Containment Laboratory for Animal Diseases Control and Prevention, Harbin, China. The details of animal experiments were described previously (Zhao et al., 2019; Chen et al., 2020). Six-week-old female mice were purchased from Changsheng company Liaoning Province and kept in the experimental animal facility at Harbin Veterinary Research Institute.