BDL operation was performed under sterile conditions. 20 female SD rats were randomly into control and BDL groups, rats were anesthetized by intraperitoneal injection of pentobarbital 50mg/kg, the abdomen was opened by a median incision, and the common bile duct was double-ligated and cut between the ligatures. Sham-operated control animals underwent similar manipulation without bile duct ligation. On the 29^th day, the animals were sacrificed, and sampling was performed to detect related indicators.