Serum biochemistry assays
Blood samples were taken from the abdominal aorta of anesthetized
animals, and the serum levels of alanine aminotransferase (ALT),
aspartate amino transferase (AST), alkaline phosphatase (ALP), total
bilirubin (TBIL), and direct bilirubin (DBIL)(Sichuan Maker
Biotechnology Co. Ltd, Chengdu, China) were measured using a standard
automatic biochemical analyzer (HITACHI 7020).