Statistical analysis
The data and statistical analysis comply with the recommendations on
experimental design and analysis in pharmacology (Curtis et al., 2018).
Experimental group size of n = 5 was predetermined based on prior
experience in evaluating for statistical significance. One-way ANOVA
followed by Bonferroni post hoc test for comparison of multiple
independent groups were used for all statistical analyses with the
GraphPad Prism version 8.0 for Windows. Statistical analysis was
undertaken only when group size was at least n = 5, where nrepresents the number of independent in vitro experiments, not
technical replicates. The post hoc test was run only if F value
achieved P < 0.05 and there was no significant variance
in homogeneity. A P value less than 0.05 was considered as
statistically significant.