Figure Legends
Figure 1. Representative chromatograms showing efavirenz (and the internal standard, efavirenz-13C6) at the lower limit of quantification (LLOQ, 25 ng/mL), low quality control (LQC, 75 ng/mL), in a patient sample (1589 ng/mL).
Figure 2. Concentration-time profiles of efavirenz in cervicovaginal fluid collected with flocked swabs and plasma. The median (range) CVS efavirenz AUC0-24h estimated from the pooled data (n = 5) was 16370 ng*h/mL (5803-22088), Cmax was 1618 ng/mL (610-2438) at a Tmax of 8.0 h (8.0-12), and Cmin was 399 ng/mL (110-981). Efavirenz CVS:plasma AUC0-24 ratio was 0.41 (0.20-0.59). Efavirenz Cmin in CVS was above the protein binding adjusted IC95 for wild-type HIV-1 of 126 ng/mL (lower dashed lines) in all five patients and but below the 470 ng/mL (upper dashed lines) trough plasma effective concentration threshold established in the ENCORE 1 study in three out of five patients.
Table 1. Linearity, accuracy (%) and precision (%) for the quantification of efavirenz in cervicovaginal swab (CVS).