Fig. 7. Effects of serelaxin on LPS-induced activation of NF-κB and PPAR-γ pathways. (A) Expression of IκB mRNA analyzed with RT-PCR. The statistical result of IκB mRNA expression. (B) Expression of p-IκB detected with Western blot. (C) The statistical result of expression of p-IκB proteins. (D) Expression of P65 mRNA analyzed with RT-PCR. The statistical result of P65 mRNA expression. (E) Expression of p65 detected with Western blot. (F) The statistical result of expression of p65 proteins. (G) Expression of P65 mRNA analyzed with RT-PCR. The statistical result of P65 mRNA expression. (H) Expression of p65 detected with Western blot. (I) The statistical result of expression of p65 proteins. Histograms represented means ± SD of relative quantification from three independent experiments. *p < 0.05 versus control group,#p < 0.05 versus LPS group. †p < 0.05 versus LPS+serelaxin group.&p < 0.05 versus LPS+serelaxin+GW group.