Cell Proliferation Assay
The effect of serelaxin on cell proliferation assay was performed according to the manufacturer’s instructions of cell counting kit-8 (CCK8). Firstly, cell suspension was inoculated in a 96-well plate at a density of 4 x 103 cells/well, three parallel wells for each group. After incubation for 24h, 10 μl of CCK-8 was added to the each well, and the cells continued to be incubate for 4 h. Absorbance values were determined at 450 nm with a microplate reader ( Tecan, Männedorf, Switzerland).