Characterization of cardiac fibroblasts.
CFs were cultured for 10 d. Phase contrast images and immunocytochemistry confirmed that CFs from all strains exhibit similar mesenchymal morphology (Figure 1A) and immunostaining with an antibody against Vimentin (green). Almost all cells expressed the fibroblast marker Vimentin (Fig.1B) negative for α-SMA, vWF or cTnT(not showed) .