Chemicals and Reagents
Serelaxin was purchased from Peprotech (Rocky Hill, NJ, USA). Dulbecco’s
modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were
purchased from GIBCO (MD, USA). Lipopolysaccharides (LPSs, Escherichia
coli 055:B5) was obtained from Sigma-Aldrich (USA). Rat IL-1β, TNF-α,
and IL-6 ELISA kits were obtained from R&D (USA), and rat IL-10 ELISA
kit was purchased from Invitrogen (USA). Rabbit polyclonal antibodies
for vimentin, α-SMA, MMP-2, MMP-9, Collagen Ⅰ and Collagen Ⅲ were
purchased from Abcam (Cambridge, MA). Rabbit polyclonal antibodies for
NF-κB p65 and phospho-NF-κB p65 (S276) were purchased from Cell
Signaling Technology (Danvers, MA, USA). Rabbit polyclonal antibodies
for phospho-IκB-α, and phosphorylated-IκB-α,PPAR-γ were purchased from
Santa Cruz Biotechnology (CA, USA). GW9662 was from Cayman Chemical (Ann
Arbor, MI, USA); Rabbit polyclonal antibody for β-actin was from
Trevigen (USA). Alexa Fluor 594 conjuncted goat anti-mouse IgG and Alexa
Fluor 488 conjuncted goat anti-rabbit were obtained from Jackson
ImmunoResearch Laboratories (West Grove, PA)
4′,6-diamidino-2-phenylindole (DAPI) was purchased from Sigma-Aldrich
(Saint Louis, MO). RNAiso Plus, PrimeScript RT MasterMix Perfect Real
Time, and SYBR Premix Ex Taq II (Perfect Real Time) were purchased from
Takara (Sangon Biotech, Shanghai).