Figure 3. Effect of eperisone on pre-developed pulmonary
fibrosis.
Mice were treated with bleomycin (BLM, 1 mg/kg) or vehicle once only on
day 0. The mice were then orally administered the indicated dose of
eperisone (Epe) once daily for 9 days (from day 10 to day 18). Pulmonary
tissue sections were prepared on day 20 and subjected to a
histopathological examination (Masson’s trichrome staining; scale bar =
500 µm) (A). The collagen-positive area was determined based on Masson’s
trichrome staining images (B). The pulmonary hydroxyproline level was
determined on day 20 (C). The total respiratory system elastance, tissue
elastance, and forced vital capacity (FVC) were measured on day 20 (D).
Values represent the mean ± SEM **P < 0.01 ; *P
< 0.05 ; NS, not significant.