Microbiome composition of adult Zeugodacus cucurbitae
Differences between the 6 wild F0 parental groups (3
from M, 3 from BP) were preliminary verified. Nearly all
F0 adult microbiomes were dominated by
Enterobacteriales, with > 99% of all reads belonging to
this Order (S2). Identified ASVs in adult microbiomes belonged to 103
different genera. More than 95% of all reads belonged to just six
genera, viz. Enterobacter (32.35%), Klebsiella (31.98%),Citrobacter (21.38%), Providencia (6.38%),Raoultella (4.9%) and Pectobacterium (1.8%). Among
these, Enterobacter , Klebsiella and Citrobacterwere identified as core genera sensu .
In general, microbiomes of adult flies were less diverse than larval
microbiomes (see below) with a mean ASV richness of 26.12 (SD = 8.12)
and mean FPD of 5.97 (SD = 1.01) for adult microbiomes. They were also
more uneven (more dominated by few abundant taxa) with an average ISI of
4.95 (SD = 2.25). No differences in any diversity metric were found
between F0 adults
from different sites or different parental groups (S3).
PERMANOVA of parental F0 adult fly microbiomes (S5)
revealed significant differences
between parental groups when
species presence/absence (Unweighted Unifrac distances) was considered
(with one out of six significant post-hoc comparisons). However, when
relative abundances were taken into account and more weight was given to
more abundant taxa (Generalized Unifrac distances), then the
F0 parental groups were not significantly different. No
significant differences between sites were found for both distances
(S4). The PCoA plots (S5), could
not resolve distinct parental clusters for both Unweighted and
Generalized Unifrac distances.