References
Ashman, T.-L., and E. A. King. 2005. Are flower-visiting ants mutualists
or antagonists? A study in a gynodioecious wild strawberry. American
Journal of Botany 92:891–895.
Ashman, T.-L., T. M. Knight, J. A. Steets, P. Amarasekare, M. Burd, D.
R. Campbell, M. R. Dudash, M. O. Johnston, S. J. Mazer, R. J. Mitchell,
M. T. Morgan, and W. G. Wilson. 2004. Pollen limitation of plant
reproduction: ecological and evolutionary causes and consequences.
Ecology 85:2408–2421.
Bartomeus, I., M. G. Park, J. Gibbs, B. N. Danforth, A. N. Lakso, and R.
Winfree. 2013. Biodiversity ensures plant-pollinator phenological
synchrony against climate change. Ecology Letters 16:1331–1338.
Bell, K. L., K. S. Burgess, J. C. Botsch, E. K. Dobbs, T. D. Read, and
B. J. Brosi. 2019. Quantitative and qualitative assessment of pollen DNA
metabarcoding using constructed species mixtures. Molecular Ecology
28:431–455.
Blüthgen, N., and A.-M. Klein. 2011. Functional complementarity and
specialisation: the role of biodiversity in plant–pollinator
interactions. Basic and Applied Ecology 12:282–291.
Bohart, G. E., and W. P. Nye. 1970. Onion pollination as affected by
different levels of pollinator activity. Utah Agricultural Research
Station Bulletin 482:3-45.
Boyle, R. M. D., and B. J. R. Philogène. 1983. The native pollinators of
an apple orchard: variations and significance. Journal of Horticultural
Science 58:355–363.
Braukmann, T. W. A., M. L. Kuzmina, J. Sills, E. V. Zakharov, and P. D.
N. Hebert. 2017. Testing the efficacy of DNA barcodes for identifying
the vascular plants of Canada. PLoS ONE 12: e0169515.
Brittain, C., C. Kremen, and A.-M. Klein. 2013. Biodiversity buffers
pollination from changes in environmental conditions. Global Change
Biology 19:540-547.
Brunet, J., Y. Zhao, and M. K. Clayton. 2019. Linking the foraging
behavior of three bee species to pollen dispersal and gene flow. PLoS
ONE 14: e0212561.
Buchmann, S., and G. P. Nabhan. 1996. The Forgotten Pollinators.
Washington D.C., Island Press/ Shearwater Books.
Campbell, J. W., J. C. Daniels, and J. D. Ellis. 2018. Fruit set and
single visit stigma pollen deposition by managed bumble bees and wild
bees in Citrullus lanatus (Cucurbitales: Cucurbitaceae). Journal
of Economic Entomology 111:989-992.
Carden, P. W., and D. J. Emmett. 1973. Artificially introduced blowflies
show promise in strawberry pollination trials. Grower 79:1017-1019.
Cardinale, B. J., J. E. Duffy, A. Gonzalez, D. U. Hooper, C. Perrings,
P. Venail, A. Narwani, G. M. Mace, D. Tilman, D. A. Wardle, A. P.
Kinzig, G. C. Daily, M. Loreau, J. B. Grace, A. Larigauderie, D. S.
Srivastava, and S. Naeem. 2012. Biodiversity loss and its impact on
humanity. Nature 486:59–67.
Clements, R. 1982. Blowflies worth considering for tunnel pollination.
Grower 97:24-26.
Cristescu, M. E. 2014. From barcoding single individuals to
metabarcoding biological communities: towards an integrative approach to
the study of global biodiversity. Trends in Ecology & Evolution
29:566–571.
Currah, L., and D. J. Ockendon. 1983. Onion pollination by blowflies and
honeybees in large cages. Annals of Applied Biology 103:497–506.
de Melo Moura, C. C., Setyaningsih, C. A., Li, K.,Merk, M. S., Schulze,
S., Raffiudin, R., Grass, I., Behling, H., Tscharntke, T., Westphal, C.,
Gailing, O. 2022. Biomonitoring via DNA metabarcoding and light
microscopy of bee pollen in rainforest transformation landscapes of
Sumatra. BMC Ecology and Evolution 22:51.
Dicks, L. V., A. Abrahams, J. Atkinson, J. Biesmeijer, N. Bourn, C.
Brown, M. J. F. Brown, C. Carvell, C. Connolly, J. E. Cresswell, P.
Croft, B. Darvill, P. De Zylva, P. Effingham, M. Fountain, A. Goggin, D.
Harding, T. Harding, C. Hartfield, M. S. Heard, R. Heathcote, D. Heaver,
J. Holland, M. Howe, B. Hughes, T. Huxley, W. E. Kunin, J. Little, C.
Mason, J. Memmott, J. Osborne, T. Pankhurst, R. J. Paxton, M. J. O.
Pocock, S. G. Potts, E. F. Power, N. E. Raine, E. Ranelagh, S. Roberts,
R. Saunders, K. Smith, R. M. Smith, P. Sutton, L. A. N. Tilley, A.
Tinsley, A. Tonhasca, A. J. Vanbergen, S. Webster, A. Wilson, and W. J.
Sutherland. 2013. Identifying key knowledge needs for evidence-based
conservation of wild insect pollinators: a collaborative cross-sectoral
exercise. Insect Conservation and Diversity 6:435–446.
Dormann, C.F., Gruber, B., Fruend, J. (2008). Introducing the bipartite
Package: Analysing Ecological Networks. R News 8(2), 8-11.
Edgar, R. C. 2010. Search and clustering orders of magnitude faster than
BLAST. Bioinformatics 26:2460–2461.
Elbrecht, V., and D. Steinke. 2018. Scaling up DNA metabarcoding for
freshwater macrozoobenthos monitoring. Freshwater Biology 64:380–387.
Folmer, O., Black, M., Hoeh, W., Lutz, R., and R, Vrijenhoek. 1994. DNA
primers for amplification of mitochondrial cytochrome c oxidase subunit
I from diverse metazoan invertebrates. Molecular Marine Biology and
Biotechnology, 3, 294–299
Fontaine, C., I. Dajoz, J. Meriguet, and M. Loreau. 2005. Functional
diversity of plant–pollinator interaction webs enhances the persistence
of plant communities. PLoS Biology 4:e1.
Free, J. B. 1966. The pollination of the beans Phaseolus
multiflorus and Phaseolus vulgaris by honeybees. Journal of
Apicultural Research 5:87–91.
Garibaldi, L. A., I. Steffan-Dewenter, R. Winfree, M. A. Aizen, R.
Bommarco, S. A. Cunningham, C. Kremen, L. G. Carvalheiro, L. D. Harder,
O. Afik, I. Bartomeus, F. Benjamin, V. Boreux, D. Cariveau, N. P.
Chacoff, J. H. Dudenhöffer, B. M. Freitas, J. Ghazoul, S. Greenleaf, J.
Hipólito, A. Holzschuh, B. Howlett, R. Isaacs, S. K. Javorek, C. M.
Kennedy, K. M. Krewenka, S. Krishnan, Y. Mandelik, M. M. Mayfield, I.
Motzke, T. Munyuli, B. A. Nault, M. Otieno, J. Petersen, G. Pisanty, S.
G. Potts, R. Rader, T. H. Ricketts, M. Rundlöf, C. L. Seymour, C.
Schüepp, H. Szentgyörgyi, H. Taki, T. Tscharntke, C. H. Vergara, B. F.
Viana, T. C. Wanger, C. Westphal, N. Williams, and A. M. Klein. 2013.
Wild pollinators enhance fruit set of crops regardless of honey bee
abundance. Science 339:1608–1611.
Garibaldi, L. A., L. G. Carvalheiro, S. D. Leonhardt, M. A. Aizen, B. R.
Blaauw, R. Isaacs, M. Kuhlmann, D. Kleijn, A. M. Klein, C. Kremen, L.
Morandin, J. Scheper, and R. Winfree. 2014. From research to action:
enhancing crop yield through wild pollinators. Frontiers in Ecology and
the Environment 12:439–447.
Garratt, M. P. D., R. Brown, C. Hartfield, A. Hart, and S. G. Potts.
2018. Integrated crop pollination to buffer spatial and temporal
variability in pollinator activity. Basic and Applied Ecology 32:77–85.
Ghazoul, J. 2005. Buzziness as usual? Questioning the global pollination
crisis. Trends in Ecology & Evolution 20:367–373.
Hebert, P. D. N., A. Cywinska, S. L. Ball, and J. R. deWaard. 2003a.
Biological identifications through DNA barcodes. Proceedings of the
Royal Society B: Biological Sciences 270:313–321.
Hebert, P.D.N., Ratnasingham, S., de Waard, J.R., 2003b. Barcoding
animal life: cytochrome c oxidase subunit 1 divergences among closely
related species. Proeedings of the Royal Society, London B Biological
Sciences 270, S96–S99.
Heinrich, B., and E. Mcclain. 1986. “Laziness” and hypothermia as a
foraging strategy in flower scarabs (Coleoptera: Scarabaeidae).
Physiological Zoology 59:273–282.
Hernández-Triana, L. M., S. W. Prosser, M. A. Rodríguez-Perez, L. G.
Chaverri, P. D. N. Hebert, and T. Ryan Gregory. 2014. Recovery of DNA
barcodes from blackfly museum specimens (Diptera: Simuliidae) using
primer sets that target a variety of sequence lengths. Molecular Ecology
Resources 14:508–518.
Hodgkiss, D., M. J. F. Brown, and M. T. Fountain. 2018. Syrphine
hoverflies are effective pollinators of commercial strawberry. Journal
of Pollination Ecology 22:55–66.
Hoehn, P., T. Tscharntke, J. M. Tylianakis, and I. Steffan-Dewenter.
2008. Functional group diversity of bee pollinators increases crop
yield. Proceedings of the Royal Society B: Biological Sciences
275:2283–2291.
Hollingsworth, P. M., L. L. Forrest, J. L. Spouge, M. Hajibabaei, S.
Ratnasingham, M. van der Bank, M. W. Chase, R. S. Cowan, D. L. Erickson,
A. J. Fazekas, S. W. Graham, K. E. James, K.-J. Kim, W. J. Kress, H.
Schneider, J. van AlphenStahl, S. C. H. Barrett, C. van den Berg, D.
Bogarin, K. S. Burgess, K. M. Cameron, M. Carine, J. Chacón, A. Clark,
J. J. Clarkson, F. Conrad, D. S. Devey, C. S. Ford, T. A. J. Hedderson,
M. L. Hollingsworth, B. C. Husband, L. J. Kelly, P. R. Kesanakurti, J.
S. Kim, Y.-D. Kim, R. Lahaye, H.-L. Lee, D. G. Long, S. Madriñán, O.
Maurin, I. Meusnier, S. G. Newmaster, C.-W. Park, D. M. Percy, G.
Petersen, J. E. Richardson, G. A. Salazar, V. Savolainen, O. Seberg, M.
J. Wilkinson, D.-K. Yi, and D. P. Little. 2009. A DNA barcode for land
plants. Proceedings of the National Academy of Sciences of the United
States of America 106:12794–12797.
Hooper, C. H. 1932. The insect visitors of fruit blossoms. Journal of
the Royal Society of Arts 81:86–105.
Inouye, D. W., B. M. H. Larson, A. Ssymank, and P. G. Kevan. 2015. Flies
and flowers III: ecology of foraging and pollination. Journal of
Pollination Ecology 16:115–133.
Ivanova, N. V., J. R. Dewaard, and P. D. N. Hebert. 2006. An
inexpensive, automation-friendly protocol for recovering high-quality
DNA. Molecular Ecology Notes 6:998–1002.
Ivanova, N. V., A. J. Fazekas, and P. D. N. Hebert. 2008.
Semi-automated, membrane-based protocol for DNA isolation from plants.
Plant Molecular Biology Reporter 26, 186-198.
Kearns, C. A., D. W. Inouye, and N. M. Waser. 1998. Endangered
mutualisms: the conservation of plant-pollinator interactions. Annual
Review of Ecology and Systematics 29:83–112.
Kearse, M., R. Moir, A. Wilson, S. Stones-Havas, M. Cheung, S. Sturrock,
S. Buxton, A. Cooper, S. Markowitz, C. Duran, T. Thierer, B. Ashton, P.
Meintjes, and A. Drummond. 2012. Geneious Basic: an integrated and
extendable desktop software platform for the organization and analysis
of sequence data. Bioinformatics 28:1647–1649.
Keller, A., N. Danner, G. Grimmer, M. Ankenbrand, K. von der Ohe, W. von
der Ohe, S. Rost, S. Härtel, and I. Steffan-Dewenter. 2015. Evaluating
multiplexed next-generation sequencing as a method in palynology for
mixed pollen samples. Plant Biology 17:558–566.
Kendall, D. A., and M. E. Solomon. 1973. Quantities of pollen on the
bodies of insects visiting apple blossom. Journal of Applied Ecology
10:627.
Kennedy, C. M., E. Lonsdorf, M. C. Neel, N. M. Williams, T. H. Ricketts,
R. Winfree, R. Bommarco, C. Brittain, A. L. Burley, D. Cariveau, L. G.
Carvalheiro, N. P. Chacoff , S. A. Cunningham, B. N. Danforth, J. H.
Dudenhoffer, E. Elle, H. R. Gaines, L. A. Garibaldi, C. Gratton, A.
Holzschuh, R. Isaacs, S. K. Javorek, S. Jha, A.-M. Klein, K. Krewenka,
Y. Mandelik, M. M. Mayfield, L. Morandin, L. A. Neame, M. Otieno, M.
Park, S. G. Potts, M. Rundlof, A. Saez, I. Steffan-Dewenter, H. Taki, B.
F. Viana, C. Westphal, J. K. Wilson, S. Greenleaf, and C. Kremen. 2013.
A global quantitative synthesis of local and landscape effects on wild
bee pollinators in agroecosystems. Ecology Letters 16:584–599.
Klatt, B., F. Klaus, C. Westphal, and T. Tscharntke. 2014. Enhancing
crop shelf life with pollination. Agriculture & Food Security 3:14.
Klein, A.-M., B. E. Vaissière, J. H. Cane, I. Steffan-Dewenter, S. A.
Cunningham, C. Kremen, and T. Tscharntke. 2007. Importance of
pollinators in changing landscapes for world crops. Proceedings of the
Royal Society B: Biological Sciences 274:303–313.
Klein, A.-M. 2011. Plant–pollinator interactions in changing
environments. Basic and Applied Ecology 12:279–281.
Kraaijeveld, K., L. A. de Weger, M. Ventayol García, H. Buermans, J.
Frank, P. S. Hiemstra, and J. T. den Dunnen. 2015. Efficient and
sensitive identification and quantification of airborne pollen using
next-generation DNA sequencing. Molecular Ecology Resources 15:8–16.
Kumar, J., R. C. Mishra, and J. K. Gupta. 1985. The effect of mode of
pollination on Allium species with observation on insects as
pollinators. Journal of Apicultural Research 24:62–66.
Kuzmina, M. L., W. A. Braukmann, A. J. Fazekas, S. W. Graham, S. L.
Dewaard, A. Rodrigues, B. A. Bennett, T. A. Dickinson, J. M. Saarela, M.
Catling, S. G. Newmaster, D. M. Percy, E. Fenneman, A. Lauron-Moreau, B.
Ford, L. Gillespie, S., J. Whitton, L. Jennings, D. Metsger, C. P.
Warne, A. Brown, E. Sears, J. R. Dewaard, E. V Zakharov, and P. D. N.
Hebert. 2017. Using herbarium-derived DNAs to assemble a large-scale DNA
barcode library for the vascular plants of Canada. Applications in Plant
Sciences 5:1700079.
Larson, B., P. G. Kevan, and D. W. Inouye. 2001. Flies and flowers:
taxonomic diversity of anthophiles and pollinators. The Canadian
Entomologist 133:439–465.
Legendre, P., and E. D. Gallagher. 2001. Ecologically meaningful
transformations for ordination of species data. Oecologia 129:271–280.
Little, D. P. 2014. A DNA mini-barcode for land plants. Molecular
Ecology Resources 14(3): 437-446.
Lopez-Medina, J., A. Palacio-Villegas, and E. Vazquez-Ortiz. 2006.
Misshaped fruit in strawberry, an agronomic evaluation. V International
Strawberry Symposium 708:77–78.
Lucas, A., O. Bodger, B. J. Brosi, C. R. Ford, D. W. Forman, C. Greig,
M. Hegarty, P. J. Neyland, and N. de Vere. 2018. Generalisation and
specialisation in hoverfly (Syrphidae) grassland pollen transport
networks revealed by DNA metabarcoding. Journal of Animal Ecology
87:1008–1021.
Martin, M. 2011. Cutadapt removes adapter sequences from high-throughput
sequencing reads. EMBnet.journal 17:10.
Morgan, K. R., and B. Heinrich. 1987. Temperature regulation in bee- and
wasp-mimicking syrphid flies. Journal of Experimental Biology 133:59-71.
Müller, A., S. Diener, S. Schnyder, K. Stutz, C. Sedivy, and S. Dorn.
2006. Quantitative pollen requirements of solitary bees: implications
for bee conservation and the evolution of bee–flower relationships.
Biological Conservation 130:604–615.
Nye, W., and J. Anderson. 1974. Insect pollinators frequenting
strawberry blossoms and the effect of honey bees on yield and fruit
quality. Journal of the American Society for Horticulture Science
99:40–44.
Oksanen, J., F. G. Blanchet, R. Kindt, P. Legendre, R. B. O’Hara, G. L.
Simpson, P. Solymos, M. H. H. Stevens, and H. Wagner. 2019. Vegan:
community ecology package.
Ollerton, J. 2017. Pollinator diversity: distribution, ecological
function, and conservation. Annual Review of Ecology, Evolution, and
Systematics 48:353–376.
Ollerton, J., R. Winfree, and S. Tarrant. 2011. How many flowering
plants are pollinated by animals? Oikos 120:321-326.
Orford, K. A., I. P. Vaughan, and J. Memmott. 2015. The forgotten flies:
the importance of non-syrphid Diptera as pollinators. Proceedings of the
Royal Society B: Biological Sciences 282:20142934.
Palmieri, L., E. Bozza, L. Giongo, L. Palmieri, E. Bozza, and L. Giongo.
2009. Soft fruit traceability in food matrices using real-time PCR.
Nutrients 1:316–328.
Pornon, A., C. Andalo, M. Burrus, and N. Escaravage. 2017. DNA
metabarcoding data unveils invisible pollination networks. Scientific
Reports 7:16828.
Potter, C., de Vere, N., Jones, L. E., Ford, C. R., Hegarty, M. J.,
Hodder, K. H., Diaz, A., Franklin E. L. 2019. Pollen metabarcoding
reveals broad and species-specific resource use by urban bees. PeerJ
7:e5999.
Rabinowitz, D., J. K. Rapp, V. L. Sork, B. J. Rathcke, G. A. Reese, and
J. C. Weaver. 1981. Phenological properties of wind- and
insect-pollinated prairie plants. Ecology 62:49–56.
Rader, R., I. Bartomeus, L.A. Garibaldi, M.P.D. Garratt, B.G.
Howlett, R. Winfree, S.A. Cunningham, M.M. Mayfield, A.D. Arthur, G.K.S.
Andersson, R. Bommarco, C. Brittain, L.G. Carvalheiro, N.P.
Chacoff, M.H. Entling, B. Foully, B.M. Freitas, B. Gemmill-Herren, J.
Ghazoul, S.R. Griffin, C.L. Gross, L. Herbertsson, F. Herzog, J.
Hipólito, S. Jaggar, F. Jauker, A-M. Klein, D. Kleijn, S. Krishnan, C.Q.
Lemos, S.A.M. Lindström, Y. Mandelik, V.M. Monteiro, W. Nelson, L.
Nilsson, D.E. Pattemore, N. de O. Pereira, G. Pisanty, S.G. Potts, M.
Reemer, M. Rundlöf, C.S. Sheffield, J. Scheper, C. Schüepp, H.G.
Smith, D.A. Stanley, J.C. Stout, H. Szentgyörgyi, H. Taki, C.H.
Vergara, B.F. Viana, and M. Woyciechowski. 2016. Non-bee insects are
important contributors to global crop pollination. Proceedings of the
National Academy of Sciences of the United States of America
113:146-151.
Rader, R., B. G. Howlett, S. A. Cunningham, D. A. Westcott, L. E.
Newstrom-Lloyd, M. K. Walker, D. A. J. Teulon, and W. Edwards. 2009.
Alternative pollinator taxa are equally efficient but not as effective
as the honeybee in a mass flowering crop. Journal of Applied Ecology
46:1080–1087.
Rahl, M. 2008. Microscopic identification and purity determination of
pollen grains. Methods in Molecular Medicine 138:263–269.
Ratnasingham, S., and P. D. N. Hebert. 2007. BOLD: the barcode of life
data system (http://www.barcodinglife.org). Molecular Ecology Notes
7:355–364.
Ratnasingham, S., and P. D. N. Hebert. 2013. A DNA-based registry for
all animal species: the barcode index number (BIN) system. PLoS ONE
8:e66213.
Richardson, R., C.-H. Lin, J. Quijia, N. Riusech, K. Goodell, and R.
Johnson. 2015. Rank-based characterization of pollen assemblages
collected by honey bees using a multi-locus metabarcoding approach.
Applications in Plant Sciences 3:1500043.
Rogers, S. R., D. R. Tarpy, and H. J. Burrack. 2014. Bee species
diversity enhances productivity and stability in a perennial crop. PLoS
ONE 9:e97307.
Russo, L. A., M. G. Park, E. J. Blitzer, and B. N. Danforth. 2017.
Flower handling behavior and abundance determine the relative
contribution of pollinators to seed set in apple orchards. Agriculture,
Ecosystems & Environment 246:102-108
Schluter, D., T. Price, and P. Grant. 1985. Ecological character
displacement in Darwin’s finches. Science 227:1056–1059.
Sheffield, C. S. 2014. Pollination, seed set and fruit quality in apple:
studies with Osmia lignaria (Hymenoptera: Megachilidae) in the
Annapolis Valley, Nova Scotia, Canada. Journal of Pollination Ecology
12:120-128.
Skevington, J. H., and P. T. Dang. 2002. Exploring the diversity of
flies (Diptera). Biodiversity 3:3–27.
Solomon, M., and D. Kendall. 1970. Pollination by the syrphid fly,Eristalis tenax . 101. [Annual Report]
Stewart, R. I. A., G. K. S. Andersson, C. Brönmark, B. K. Klatt, L.-A.
Hansson, V. Zülsdorff, and H. G. Smith. 2017. Ecosystem services across
the aquatic–terrestrial boundary: linking ponds to pollination. Basic
and Applied Ecology 18:13–20.
Tan, G.-M., L. Xu, D.-B. Bu, S.-Z. Feng, and N.-H. Sun. 2006.
Improvement of performance of MegaBlast algorithm for DNA sequence
alignment. Journal of Computer Science and Technology 21:973–978.
Tennekes, M. 2022. Treemap: Treemap Visualization. R package.https://CRAN.R-project.org/package=treemap
Ver Hoef, J. M., and P. L. Boveng. 2007. Quasi-poisson vs. negative
binomial regression: how should we model overdispersed count data?
Ecology 88:2766–2772.
Wardhaugh, C. W. 2015. How many species of arthropods visit flowers?
Arthropod-Plant Interactions 9:547–565.
Woodcock, T. S. 2012. Pollination in the agricultural landscape best
management practices for crop pollination. Canadian Pollination
Initiative (NSERC-CANPOLIN), University of Guelph report. 113 pp.