2.1. Production of Force-Degradation Samples
Many factors affect the stability of antibodies during the development of therapeutic proteins, such as temperature, light, and other factors. The anti-vascular endothelial growth factor (VEGF) antibodies concentration were 25mg/mL and were kept in our laboratory. The product was treated with exposure to the sunlight for 12, 24, 48, and 96 h and heat at 60°C in a metal bath for 12, 24, 48, and 96 h under diverse conditions. Unprocessed samples were used as positive controls. All samples were in solution.