2.1. Production of Force-Degradation Samples
Many factors affect the stability of antibodies during the development
of therapeutic proteins, such as temperature, light, and other factors.
The anti-vascular endothelial
growth factor (VEGF) antibodies concentration were 25mg/mL and were kept
in our laboratory. The product was treated with exposure to the sunlight
for 12, 24, 48, and 96 h and heat at 60°C in a metal bath for 12, 24,
48, and 96 h under diverse conditions. Unprocessed samples were used as
positive controls. All samples were in solution.