2.7. Raman Spectra Instrument and Settings
The Raman spectra were measured using TruScan™ RM (TSRM) Handheld Raman
analyzers equipped with the embedded TruTools™ chemometrics software
package (Thermo Fisher Scientific). The device is a direct dispersive
Raman that uses a 785-nm laser excitation wavelength. Its average
spectral resolution varies between 8 and 10.5
cm−1, and the Raman
shift range is 250–2875 cm−1. The mAb product
solutions were transferred to 4-mL glass vials, which served as the
sample cells for Raman spectrum acquisition. The acquisition was
executed in the vial holder to prevent interference from ambient light.
The excitation used identical spectral acquisition settings: 250 mW,
5000 ms, and 3 coadds.