Figure 3. Validation of the RGA bioassay. (a ) Four
different sample concentrations were seeded to be inhibited by the VEGF
(primary concentration: 100, 95, 90, and
75 µg/mL; dilution ratio: 1:4). The
inhibition curves of the sample are shown. (b ) The primary
concentration for 12-h light treatment, 24-h light treatment, and
positive control was 100 µg/mL; the primary concentration of the 50%
sample was 50 µg/mL; dilutions of 1:4 were used to construct four
inhibition curves; (c ) The primary concentration for 48-h light
treatment, 96-h light treatment, 12-h heat treatment, and positive
control was 100 µg/mL; dilutions of 1:4 were used to construct four
inhibition curves; (d ) The primary concentration for 24-h heat
treatment, 48-h heat treatment, 96-h heat treatment, and positive
control was 100 µg/mL; dilutions of 1:4 were used to construct four
inhibition curves. RGA, reporter gene assay; VEGF, anti-vascular
endothelial growth factor.
The bioassay results for the bioactivity were successfully evaluated by
RGA.The results of the bioactivity are shown in Table 4.
Table 4. The result of the bioacitivity.