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\begin{document}

\title{A simple~ way to synthesize {[}\textsuperscript{18}F{]}flumazenil for
clinics}



\author[1]{SRSTT Radiopharmaconnect}%
\affil[1]{Radiopharmaconnect}%


\vspace{-1em}



  
  \date{May 25, 2024}


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\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/CAFACHEM1/CAFACHEM1}

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\end{figure}

\textbf{Authors: Pardeep Kumar\textsuperscript{1}}, Raman Kumar
Joshi\textsuperscript{1}, Chandana Nagaraj\textsuperscript{1}, N.
Sridhar Goud\textsuperscript{1}, Naren P Rao\textsuperscript{2} ~

Department of Neuroimaging and Interventional
Radiology\textsuperscript{1},

Psychiatry\textsuperscript{2}

\par\null

\textbf{Background}

Fluorine-18 {[}\textsuperscript{18}F{]}flumazenil (FMZ) has been used
for the assessment of the gamma amino butyric acid (GABA) receptors by
positron emission tomography (PET). {[}\textsuperscript{11}C{]} or
{[}\textsuperscript{18}F{]}flumazenil have been used for PET imaging of
various types of neurodisorders. ~

\par\null

\textbf{Aims}

To standardize and synthesize {[}\textsuperscript{18}F{]}flumazenil
using various combinations of the solid phase cartridges in order to
minimize the loss of radiochemical yield during purification.

\par\null

\textbf{Methods}

The precursor nitormazenil was procured from Syncom BV, Netherlands. The
radio-fluorination was standardized for various parameters like
temperature (130-160\selectlanguage{ngerman}°C), precursor concentration (3-5 mg), various
combination of SPE cartridges (HLB, tC18, C18 and alumina) and final
product eluted by using 2.0 mL of 20\% ethanol/ phosphate buffer
(pH-4.0). The radiochemical purity was calculated by using Radio-HPLC.

\par\null

\textbf{Results and Conclusion}

The combination of C18 and alumina cartridges gave the highest yield (15
± 2 \%) as compare to 1-2\% by other two combination. The radiochemical
purity was 95 ± 3 \% with retention time at 15.4 ± 0.3 min (n = 10).
{[}\textsuperscript{18}F{]}flumazenil was synthesized with higher
radiochemical purity and a simple automated method has been developed
for the clinical use.\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/1/1}
\caption{{Fig 1. showing uptake pattern of {[}\textsuperscript{18}F{]}flumazenil
({[}\textsuperscript{18}F{]}FMZ) in the volunteer brain. The regional
time activity curve showed the highest uptake in the neocortical
regions, followed by cerebellum, thalamus and putamen, and lowest uptake
in the pons ~
{\label{840776}}%
}}
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\includegraphics[width=0.70\columnwidth]{figures/CAFACHEM/CAFACHEM}

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\end{figure}

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