Feline panleukopenia virus associated Hypogranular cerebellar hypoplasia
in young captive lions
Abstract
Here we described brain lesion in young captive lions after exhibiting
ataxia. The histopathological analyses of the brain showed typical
encephalitis with glial cell proliferation, neuronal atrophy and
necrosis, and encephalomalacia. We used a variety of identification
methods, including next-generation sequencing, polymerase chain reaction
(PCR), virus isolation, immunofluorescence staining (IFA), and electron
microscopy. Combined with clinical manifestations of the infected cub,
it determined that the dead cub have resulted from an FPV embryonic
infection. Through sequence analysis, it was found that the infected
virus may be a recombinant virus between FPV and CPV. this study not
only provides new gene sequences for the global study of FPV-infected
lions but also helps to further study the incidence and genetic
diversity of FPV.